Yes. The spouse of an L-1 transferee is employment-authorized incident to that status, which is a materially different position from the dependants of a professional admission, who may not work at all. Children under twenty-one accompany as derivatives and may attend school.
The spouse's position depends entirely on the principal's category
Households comparing routes should weigh this deliberately, because it is often the difference that decides where a family can actually afford to live. Under an intracompany transfer the spouse's ability to work follows the status itself, so a second career does not have to be suspended for the duration of the assignment. Two cautions belong with that.
The permission belongs to a spouse in a legally recognised marriage; a partner who is not married to the principal is outside the derivative scheme altogether and needs an independent basis. And derivative children age out at twenty-one, which for a long assignment means a teenager who arrives comfortably inside the limit may need their own status before the family expects it. If either point applies, plan the alternative before the move rather than after.